Combining Ability of Callus Induction and Plant Regeneration in Sorghum Anther Culture

diallel analysis of callus induction and plant regeneration in rice anther culture

low efficiency of callus induction and green-plant regeneration have limited the application of anther culture in breeding programs. this study was conducted to determine the inheritance of callus induction and plant regeneration in rice (oryza sativa) anther culture. ten genotypes drived from a five-parent diallel mating set contain khazar, sepidrood, hashemi, alikazemi and nematt were evaluat...

Regeneration of Stevia Plant Through Callus Culture

Stevia rebaudiana Bertoni that conventionally propagated by seed or by cuttings or clump division which has a limitation of quality and quantity seed material. In present study, callus culture technique was tried to achieve rapid plant multiplication for quality seed material. Callus induction and multiplication medium was standardized from nodal as well as leaf sagments. It is possible to main...

Evaluation of callus induction and plant regeneration in Citrullus colocynthis (L.) Schrad. Sormeh Gharehmatrossian*

In the present study, small segments of cotyledon, apical bud, hypocotyl, and root of 10-day-old seedlings were isolated and transferred to Murashige and Skoog (MS) base medium with different treatments of phytohormones. Two media were used for apical bud culture: MS with indole-3-acetic acid (IAA) and kinetin (Kin) (1 mg/l) and the other medium, MS with double vitamin of MS and 6-benzylaminopu...

Callus culture and regeneration.

In vitro Callus Induction and Rosmarinic Acid Quantification in Callus Culture of Satureja khuzistanica Jamzad (Lamiaceae)

In the present study, an efficient protocol has been developed for callus induction and production of RA in callus culture of Satureja khuzistanica for the first time. In vitro callus induction was achieved from young shoot tip explants cultured on MS and B5 media supplemented with different concentrations of IBA (0.1, 1.0, 2.0 and 5.0 mgl-1) solely or in combination with cytokinins BAP and KIN...